Academic Thesis
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Basic information
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| Name | SATO Kazuomi |
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| researchmap researcher code | 6000019641 |
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Indomethacin inhibits melanogenesis via down-regulation of Mitf mRNA transcription.
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Joint Author
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Kazuomi Sato, Masahiro Takei, Ray Iyota, Yoshimasa Muraoka, Marika Nagashima, Yoshitaka Yoshimura
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Non-steroidal anti-inflammatory drugs (NSAIDs) exhibit several divergent biological effects. In this study, we investigated the effect of indomethacin on melanin synthesis using B16F1 melanoma cells. Indomethacin inhibited α-melanocyte stimulating hormone (α-MSH)-enhanced melanin synthesis in a dose-dependent manner. Western blotting analysis revealed that indomethacin significantly suppressed tyrosinase and Mitf protein levels. In a luciferase reporter assay, we found that indomethacin reduced tyrosinase promoter activity. Moreover, real-time RT-PCR analysis showed that indomethacin lowered mRNA levels of melanogenic genes, including Mitf. Together, our findings indicate that indomethacin inhibits melanogenesis via the suppression of Mitf transcription.
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Bioscience, Biotechnology, and Biochemistry
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Taylor & Francis
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81
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12
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2307
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～
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2313
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2017
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Exist
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Not exist
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English
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Research papers (academic journals)
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International
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0916-8451(print) 1347-6947 (online)
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0916-8451(print) 1347-6947 (online)
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10.1080/09168451.2017.1394812
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29090638
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Url
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