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基本情報
| 氏名 |
佐藤 一臣 |
| 氏名（カナ） |
サトウ カズオミ |
| 氏名（英語） |
SATO Kazuomi |
| 所属 |
農学部 生産農学科 |
| 職名 |
教授 |
| researchmap研究者コード |
6000019641 |
| researchmap機関 |
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Indomethacin inhibits melanogenesis via down-regulation of Mitf mRNA transcription.
Kazuomi Sato, Masahiro Takei, Ray Iyota, Yoshimasa Muraoka, Marika Nagashima, Yoshitaka Yoshimura
Non-steroidal anti-inflammatory drugs (NSAIDs) exhibit several divergent biological effects. In this study, we investigated the effect of indomethacin on melanin synthesis using B16F1 melanoma cells. Indomethacin inhibited α-melanocyte stimulating hormone (α-MSH)-enhanced melanin synthesis in a dose-dependent manner. Western blotting analysis revealed that indomethacin significantly suppressed tyrosinase and Mitf protein levels. In a luciferase reporter assay, we found that indomethacin reduced tyrosinase promoter activity. Moreover, real-time RT-PCR analysis showed that indomethacin lowered mRNA levels of melanogenic genes, including Mitf. Together, our findings indicate that indomethacin inhibits melanogenesis via the suppression of Mitf transcription.
Bioscience, Biotechnology, and Biochemistry
0916-8451(print) 1347-6947 (online)
0916-8451(print) 1347-6947 (online)
10.1080/09168451.2017.1394812
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